cccd7c41-205f-4a5c-bbc9-328ba3dbf59620230103074400669wseas:wseasmdt@crossref.orgMDT DepositWSEAS TRANSACTIONS ON ENVIRONMENT AND DEVELOPMENT2224-34961790-507910.37394/232015http://wseas.org/wseas/cms.action?id=40311420221420221810.37394/232015.2022.18https://wseas.com/journals/ead/2022.phpPatrizia ,BarbaniDepartment of Civil and Industrial Engineering, University of Pisa, Largo Lucio Lazzarino 2, 56122, ITALYNiccolettaBarbaniDepartment of Civil and Industrial Engineering, University of Pisa, Largo Lucio Lazzarino 2, 56122, ITALYSaraFilippiDepartment of Civil and Industrial Engineering, University of Pisa, Largo Lucio Lazzarino 2, 56122, ITALYGiovannaStrangisDepartment of Civil and Industrial Engineering, University of Pisa, Largo Lucio Lazzarino 2, 56122, ITALYMarcoSandroniDepartment of Civil and Industrial Engineering, University of Pisa, Largo Lucio Lazzarino 2, 56122, ITALYAntonioPratelliDepartment of Civil and Industrial Engineering, University of Pisa, Largo Lucio Lazzarino 2, 56122, ITALYMaria JLopezUnit of Microbiology, Department of Biology and Geology, CIAIMBITAL, ceiA3, University of Almeria, Almeria, 04120, SPAINPabloBarrancoUnit of Microbiology, Department of Biology and Geology, CIAIMBITAL, ceiA3, University of Almeria, Almeria, 04120, SPAINTomasCabelloUnit of Microbiology, Department of Biology and Geology, CIAIMBITAL, ceiA3, University of Almeria, Almeria, 04120, SPAINPatriciaCastilloUnit of Microbiology, Department of Biology and Geology, CIAIMBITAL, ceiA3, University of Almeria, Almeria, 04120, SPAINMarie AlinePierrardIDELUX Environment, Drève de l’arc-en-ciel 98, 6700 Arlon, BELGIUMMauriziaSeggianiDepartment of Civil and Industrial Engineering, University of Pisa, Largo Lucio Lazzarino 2, 56122, ITALYAt present just about 30% of the waste plastic collected is efficiently recycled, while the rest is incinerated, disposed in landfills, or can end up in compost and be released in the environment, inducing a very negative effect on safety and health of flora and fauna. Sustainable management of hardly recyclable plastic waste generated by light weight single use packaging and agricultural films can be improved by applying biotechnological approaches, combining microorganisms, new enzymes, earthworms, and insects to work collaboratively, not only to promote the degradation of these plastics but also to obtain, by-products of the biodegradation process to be valorized as fertilizers, functional polysaccharides, etc. In order to develop a feasible process, mapping and characterization of the most diffused agri-food waste plastic were conducted isolating the main types of plastic involved. Plastic waste in agriculture is mainly constituted by polyethylene (PE) both linear low density (LLDPE) and high density (HDPE), polypropylene (PP) and polystyrene (PS), whereas in food packaging polyethylene is still present together with a large presence of polypropylene, polystyrene and polyethylene terephthalate (PET). Combining plastic presence and availability of organisms for their degradability, representative samples of plastics (PE, PET, PS) were selected for analysis of deterioration and potential subsequent biodegradation by enzymes and organisms. To monitor the plastic degradability by enzymes, and larvae, methods for the plastic analysis were set, outlining some differences in virgin and post consumer plastic in particular after use in agriculture, assessing the possibility to monitor the degradability of plastic with time and different treatments, in particular, some evidence of polyethylene degradability from larvae of Tenebrio molitor was observed.123020221230202212761282120https://wseas.com/journals/ead/2022/c485115-038(2022).pdf10.37394/232015.2022.18.120https://wseas.com/journals/ead/2022/c485115-038(2022).pdf10.1016/j.jhazmat.2017.10.014Hahladakis, J.N., Velis, C.A., Weber, R., Iacovidou, E., Purnell P. An overview of chemical additives present in plastics: Migration, release, fate and environmental impact during their use, disposal and recycling. J. Hazard. Materials, Vol. 344, 2018, pp.179-199. Plasticseurope, Plastic the Fact, plasticseurope.org/wpcontent/uploads/2021/10/2019-Plastics-thefacts.pdf, access 11.10.2022 10.4081/jae.2011.28Scarascia Mugnozza, G., Sica, C., Russo, G., Plastic materials in European agriculture: actual use and perspectives. J. Agric. Engin,.Vol. 42, No3, 2011, pp. 15-28. 10.12775/eip.2020.001Baran, B. Plastic waste as a challenge for sustainable development and circularity in the European Union. Ekonomia i Prawo. Economics and Law, Vol. 19, No 1, 2020, pp.7-20. 10.1016/j.eti.2019.100352Laskar N., Kumar U., Plastics and microplastics: A threat to environment, Environmental Technology & Innovation Vol.14, No9, 2019, https://doi.org/10.1016/j.eti.2019.100352 Padovani G., Carlozzi P., Seggiani M, Cinelli P., Vitolo S., Lazzeri A, PHB-Rich Biomass and BioH2 Production by Means of Photosynthetic Microorganisms, Chem. Eng.Trans. Vol.49, 2016, pp. 55-60, DOI:10.3303/CET1649010 Seggiani M., Cinelli P., Geicu M., Popa Mona E., Puccini M., Lazzeri A., Microbiological Valorisation of Bio-composites Based on Polylactic Acid and Wood Fibre, Chem. Eng.Trans. Vol.49, 2016, pp. 127-132. 10.37394/232015.2022.18.41Hamitouche F., Lardjane N., Grohens Y., Habchi Laribi H., Belhaneche Bensemra N., "Natural Weathering of Plasticized Polyvinyl Chloride (PVC) Stabilized with Epoxidized Sunflower Oil," WSEAS Transactions on Environment and Development, Vol.18, 2022, pp. 417-426. 10.1016/s0141-3910(03)00129-0Bonhomme S., Cuer A., Delort A.M., Lemaire J., Sancelme M., Scott C., Environmental biodegradation of polyethylene., Polym. Degrad. Stab. Vol. 81, 2003, pp. 441-452. 10.1021/es504038aYang J., Yang Y., Wu W.M., Zhao J., Jiang L., Evidence of polyethylene biodegradation by bacterial Strains from the guts of plastic-eating waxworms”, Environ. Sci. Tech.; Vol. 48: 2014, pp.13776-13784. 10.1016/j.cub.2017.02.060Bombelli P., Howe C.J., Bertocchini F., 2017, Polyethylene bio-degradation by caterpillars of the wax moth Galleria mellonella, Current Biology, Vol. 27, No 8, pp. R292-R293. 10.1126/science.aad6359Yoshida S., Hiraga K., Takehana T., Taniguchi I., Yamaji H., Maeda Y., Toyohara K., Miyamoto K., Kimura Y., Oda K., A bacterium that degrades and assimilates poly(ethylene terephthalate), Science, Vol. 351, 2016, pp. 1196-1199. 10.1016/j.scitotenv.2020.139521Billen P., Khalifa L., Fenno Van Gerven, Tavernier S., Spatari S., Technological application potential of polyethylene and polystyrene biodegradation by macro-organisms such as mealworms and wax moth larvae, Science of The Total Environment, Vol. 735, No. 139521, 2020, , 10pp. 10.1021/acs.est.5b02661Yang Y., Yang J., Wu W.M., Zhao J., Song Y., Gao L., Yang R., Jiang L., Biodegradation and Mineralization of Polystyrene by Plastic-Eating Mealworms: Part 1. Chemical and Physical Characterization and Isotopic Tests, Environ. Sci. Technol., Vol.49, No.20, 2015, pp. 12080-12086. 10.3390/ma12111746Li D., Zhou L., Wang X., Young X., Effect of Crystallinity of Polyethylene with Different Densities on Breakdown Strength and Conductance Property, Materials, Vol. 12, 2019, pp 1746-1759; doi:10.3390/ma12111746. 10.1007/bf00654051Woo E. M., Ko T.Y., A differential scanning calorimetry study on poly(ethylene terephthalate) isothermally crystallized at stepwise temperatures: multiple melting behavior re-investigated, Colloid and Polymer Science, Vol. 274, 1996, pp 309–315.